I need strains of Agrobacterium rhizogenes or Agrobacterium tumefaciens, how can I get them?

I need the above strains for my students .

02 October 2020 5,867 5 View

What is the top ten scientific journals in the world, and how quality is evaluated?

Do quality is evaluated just depends on the impact factor (IF)? what is the greatest number of the IF?

18 July 2020 6,135 44 View

What is the main reason of global warming? Do CO2 is responsible only? Do wildfire an auto event?

The average temperature of the Earth is rising at nearly twice the rate it was 50 years ago. This rapid warming trend cannot be explained by natural cycles alone, scientists have concluded. The...

08 June 2020 3,764 38 View

Who can help me by giving some lectures about Agriculture projects?

I really need to some lectures are relationship to agriculture projects, it will be by PPT, PDF and any thing.

03 October 2019 766 5 View

How to get data from a tree node for wavelet package in matlab?

I use the function (wpdec2) in wavelet packet

28 April 2019 1,672 2 View

Do I have the appropriate sonicator?

I am following the catalyst preparation procedure expressed in the attached paper [Applied Catalysis B: Environmental 239 (2018) 280-289]. The procedure requires sonicating

14 October 2018 8,513 2 View

Can I use the enzyme 6PGD Kit that purified from mice for determination the 6PGD in human?

Estimation of enzyme activity

14 August 2018 3,460 2 View

What are the major problems related to computer networks to simulate and resolve by using OPNET

OPNET Simulator , I need a problem to make a PhD proposal by using OPNET please?

31 July 2017 2,558 2 View

Hello How to get a producer to dissolve cellulose fiber by carbon disulfide ?

11 April 2017 9,899 2 View

How can I request the stress intensity factor for a growing crack in Abaqus?

31 December 2016 5,416 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View