I intend to do Isolation of bacterial and fungal endophytic microorganisms from leaves collected from several sites. Is it possible to collect the leaf samples, store them at 4 degrees Celsius and do the isolations at a later stage.
Hello,, here the procedure how to performed isolation. You can keep leaves after cleaning process in PCB about 30days. But better to go with fresh as already mentioned above.
https://www.google.com/search?client=ms-android-google&sxsrf=ACYBGNTE7ErxAzHyWhq3dke9-xRK9IRmjQ:1580954773084&q=isolation+of+endophylic+bacteria+frol+leaves&nfpr=1&sa=X&ved=2ahUKEwijjp-967vnAhXCUn0KHSfYASgQvgUoAXoECAoQAw&biw=393&bih=658#imgrc=iBuuWqegePD4EM:
Hello,
For isolation of endophytic microbes, it’s better to use fresh sample of leaves.
If you keep your samples on the refrigerator at 4 degree, some saprophytes might be grow and suppress the endophytes
so do your isolation process as soon as possible
I agree with Wiwiek Harsonowati It must be minus 18 degrees C to suppress contamination, but fresh is best!
Hello,, here the procedure how to performed isolation. You can keep leaves after cleaning process in PCB about 30days. But better to go with fresh as already mentioned above.
https://www.google.com/search?client=ms-android-google&sxsrf=ACYBGNTE7ErxAzHyWhq3dke9-xRK9IRmjQ:1580954773084&q=isolation+of+endophylic+bacteria+frol+leaves&nfpr=1&sa=X&ved=2ahUKEwijjp-967vnAhXCUn0KHSfYASgQvgUoAXoECAoQAw&biw=393&bih=658#imgrc=iBuuWqegePD4EM:
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