I WISH TO PROFILE SOME MEDICINAL PLANTS USING THE TWO PCR METHODS MENTIONED ABOVE (RAPD & SSR)AND I NEED PRIMERS BUT I AM NOT TOO SURE OF THE SEQUENCE OF THE PRIMERS. FOR VERNONIA AMYGDALINA I HAVE GOT A SEQUENCE OF PRIMERS BUT FOR THE SSR, I DO NOT HAVE AN IDEA OF HOW TO GO ABOUT IT.
For the RAPD analysis, the primers are random and can be obtain from Operon and British columbia technologies.
You can also design the primers using oligo software with length 10 -15 bp. but note that the RAPD primers lacks reproducibility and as such are not reliable.
for the microsatellite (SSR) primers, you can design the primers for di or tri-nucloetides of between 25 - 30 bp. You can also choose random SSR primers from the database.
Dear Okwuchi Ugoanyanwu
1. SSRs are unique an you need known about their genome location so first you have to search to be sure about SSR development. If any SSRs not develop for your plants you should develop one or use ISSR that is a random marker.
2. RAPD no need to develop so you can use it but I suggest don't use that because is not reliable.
Thank you all for the answers, I will try to get the sequence information for the plants and where I can't I will have to use ISSR .
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