Algebraic Loop Problem in PV array Solution?

I am doing a project with PV array in Simulink. When I run small section of the total system i do not get an error. But when i integrate full system(my system model`s are completely okay) I get an...

18 January 2021 685 10 View

How to adapt 280 nm absorbance for protein quantification to microplates ?

Hello. I want to adapt the 280 nm absorbance method for protein quantification of cell lysates to the microplate set up. I learned that for 1 cm quartz cuvettes a conversion factor of 1.3 is used...

06 July 2020 1,818 3 View

Preventing polyhistidine tag in Plasmid from expressing

05 August 2017 5,700 3 View

Does anyone know a transparent polymer material which can sustain at high temperature ?

The material should be cost effective. I want to use those material to coat my pyrolysis reactor.

27 June 2016 1,727 8 View

Immunoblotting CD63 in exosomal proteins?

I'm having trouble in immunoblotting(western) CD63 especially in exosomal proteins, since the antibody works well in non reducing conditions. My proteins were in RLN buffer. Is this going to...

02 February 2014 295 4 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

For primer efficiency calculation can we use positive control(in which gene is abundantly expressed) and perform the analysis?

My sample of interest is sperm RNA(less abundant and highly fragmented RNA). In that case, to determine the primer efficiency, can i use tissue samples (in which the gene is abundantly expressed)...

11 November 2020 758 3 View

Hello, How long can rat sperm survive in saline solution after leaving from the testis after sacrification?

How should I measure motility, what is the process? Should I put it in the incubator (37 C) immediately after sacrification? Thank you from now.

28 October 2020 280 4 View

Checking purity of sperm suspension?

I have sperm cells extracted from epididymis of a mouse. I would like to check and quantify their purity. My first idea is to use an intercalating dye like Hoechst and then see what fraction of...

25 October 2020 8,600 1 View

Can RG Members Comment on whether Infertility in Men is associated with Tight Pants versus Loose Pants?

Male Infertility is a major public health concern worldwide and there had been heated debates to date on the above question; in other words is Boxers vs. Briefs related to Increasing Sperm Count...

21 September 2020 2,398 12 View

How to stop sperm cells clumping during fixation?

I've been optimising a flow cytometry protocol to assess intracellular proteins in human sperm. Most published methods describe fixing with 4%PFA after washing and pelleting the cells. I am...

06 September 2020 2,914 2 View

Interaction-effect with categorical dependent variable in SPSS?

Hi there, I am struggling with my thesis. I need to test in SPSS if there is a significant interaction effect. I have four groups, and they all fill in a survey. The only difference of the...

27 July 2020 6,074 1 View

I want measure intracellular pH change in sperm with BCECF-AM. It requires pH standard?

This is my first time to try measure intracellular pH but it is confusing about protocol. I have BCECF/AM (216254, CAS 117464-70-7, Sigma) and i understood protocol briefly as below. 1. Load...

04 June 2020 6,392 6 View

What software should I use for cryobiologic image analysis?

I am a PhD student I am studying varying cryopreservation methods for sperm. As part of this project I have used Cryo-scanning electron microscopy to investigate varying ice-crystal formation...

16 April 2020 9,071 2 View

Can a plant extract be added with PBS?

Can a plant extract be added with PBS for improving sperm parameters? Please reply me ASAP.

02 March 2020 4,584 2 View