Recently, our Laboratory has been culturing Atopobium vaginae (BAA-55) purchased from ATCC®. The bacteria cultured in Trypic Soy Broth and Trypic Soy Agar supplemented with 5% defibrinated sheep blood. All conducted under strict anaerobic conditions. Our cultivation methods involve both an anaerobic chamber and the use of Anaeropack Gaspack (MGC). However, we encountered a challenge after the initial subculture: we were unable to quantify bacterial colonies using the CFU method, and the OD600 value also remained below 1. Have any fellow researchers had similar experiences with culturing Atopobium vaginae? We are actively seeking insights and suggestions on how to enhance cell viability in this context. Your contributions and guidance would be greatly appreciated. Thank you in advance for your assistance.
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