Looking for anyone with experience in assessment of differences between linear construct design versus plasmid based, STARR-Seq method...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690393/?
Please see Comparative analysis of enzymatically produced novel linear DNA constructs with plasmids for use as DNA vaccines
Adam A. Walters, PhD, Ekaterina Kinnear, MSc, [...], and John S. Tregoning, PhD
Genomic DNA is digested with a restriction enzyme, end labeled with biotin-dNTP with TdT, and then immunoprecipitated with Protein G Dynabeads coated with anti-biotin antibody.
11 December 2014 9,879 15 View
The subsequent step with Klenow exo- is done at 37 degrees to adenylate the blunt ended fragments. End repair uses a cocktail of T4 DNA polymerase, Klenow, and polynucleotide kinase.
11 December 2013 7,147 7 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
02 March 2021 3,060 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View