I am trying to assess the effects of a compound as a radiosensitizer, and am interested to see if the compound aids in induction of apoptosis following radiation.
Thanks. I am doing in vitro studies, and am using 5 Gy of radiation. I waited 4 hours after radiation, and proceeded with Annexin V/ PI staining. Although I do see an increase in my drug treated samples, I have decided to give it a try with 24 hours post IR. Thanks for your comment.