Hello there,

I am currently work to remove fat from a whey protein concentrate with a fat content of ~10 % w/w in order to obtain a defatted powder. I used the folch method but instead of cloroform/methanol I used Hexane to remove apolar lipid followed by Hexane/isopropanol 3:2 for polar lipid.

After the extraction of fat the powder resulted in fine dust with little flakes (before was clumpy).

To validate the procedure, I analysed the sample with rose-gotlieb method without issues during extraction, but when I distillate the solvent for recovery to obtain only the fat in the flash it formed a coagulation similar to egg-white. It only happen when I applyed fat extraction to the powder, indeed with regular powder with fat no issues were visible during distillation and recovery of solvent.

Thanks to everyone who is interested on this.

Regards,

Giovanni