Hi Community,
I was wondering if anyone knows of a fluorescent probe that can be used to stain carbon nano tubes (CNT) that contain DNA, to track the movement of these tubes as they move into living plant cells? The process of functionalization for DNA delivery is done in three stages so there are possibly 3 forms of CNTs that can be used. A) Carboxylated CNTs solubilized with sonication; B) EDS/NHS activated CNTs [reacts with amine-linked groups]; C) CNTs functionalized with PEI (polyethylenimine 25k branched chain). Any suggestions on stains that might work is greatly appreciated.
Check on the followings:
Anal. Methods, 2020,12, 1415-1429
ACS Nano. 2008; 2(10): 2023–2028
DOI: 10.1038/srep01473
Nat. Nanotechnol. 2019, DOI: 10.1038/s41565-019-0382-5
Fluorescent Probes | Thermo Fisher Scientific - SA
Dear Yoichiro Watanabe many thanks for your very interesting technical question. In addition to the valuable references suggested by Riaz A. Khan please also have a look at the following potentially useful articles:
Fluorescent Single-Walled Carbon Nanotubes for Protein Detection
Article Fluorescent Single-Walled Carbon Nanotubes for Protein Detection
A cell nanoinjector based on carbon nanotubes
https://www.pnas.org/content/104/20/8218
and
Carbon Nanotubes as Molecular Transporters for Walled Plant Cells
Article Carbon Nanotubes as Molecular Transporters for Walled Plant Cells
Only the first article has been posted as public full text on RG and can be freely downloaded as pdf file.
Good luck with your research! 👍
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