Dear all,
I am searching for a possible (any) commercial fluorescent enzyme and its fluorescent enzymatic product (directly fluorescent or indirectly detected).
Thanks a lot in advance ;)
Hi Rafael, you will have to be a whole lot more specific.
There are literally tons of fluorescent enzyme substrates and/or products on the market.
Most proteins are fluorescent due to the fluorescence of their tryptophan residues. Also, some enzyme cofactors are fluorescent, such as flavins, pyridoxal phosphate, and NAD(P)H.
Thank you for the advice and sorry for not being so specific.
In short, I am searching for any enzyme that is fluorescently-labeled and that it catalyzes a product that could also be fluorescently detected for microscopy imaging. For that reason, naturally fluorescent molecules are mostly not suitable.
Ideally, the reaction should be simple (not many co-factors required), and both the enzyme and product's optical properties should be suitable for fluorescence microscopy.
OK, you could use dye quenched gelatin. The gelatin is so heavily labeled with fluorescein that the fluorescence is quenched. It only fluoresces when it is cleaved by a proteinase.
https://www.thermofisher.com/order/catalog/product/D12054
You could rhodamine label trypsin (or other proteinases) yourself or there might be some company out there that sells them.
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