We are going to run an assay in which a Fluorescein-labelled plasmid is spotted on a hydrogel (3% polyacrylamide) coated slides and then imaged. I was wondering how sensitive the final fluorescence will be to dryness of a hydrogel - if the hydrogel gets dry for some time in the course of the experiment, or if it gets dry during imaging - is it going to affect the fluorescence? Thanks for your responses!
Todd Martinsky
hydrogel will dry as you scan it and the data will likely change each time through the microarray scanner. Generally hydrogel type slides are very sensitive and as they dry so does the data. I would suggest epoxy 2 D surface that will covalently bind your plasmid and will have lower background than hydrogel.
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