what are the current achievable standards in terms of lower detection limit in fluorescence based assays?
Hello, Usually Fluorescence assays do not have normally defined LOD as well known analytical methods but several researchers use below formula. Here no possibility to measure signal 3time higher or lover to noise like in chromatography so use below formula for LOD.
LOD = 3 SD+ N, where N is the mean blank signal (substance concentration is virtually 0) and SD is the standard deviation for the mean blank signal.
Hope this info will help you.
Good luck.
Generally always it will be a conc. that gives a signal 3- times higher than the noise signal.
Regards
G
Hellow Arti
We can calculate the LoD in several ways. The easy way is 3*SD of blank samples. Except that techniques we can calculate LoD by 3.3*(s/b) when S is SD of blank and b is calibration graph slope. Best wishes for your future work
Hello, Usually Fluorescence assays do not have normally defined LOD as well known analytical methods but several researchers use below formula. Here no possibility to measure signal 3time higher or lover to noise like in chromatography so use below formula for LOD.
LOD = 3 SD+ N, where N is the mean blank signal (substance concentration is virtually 0) and SD is the standard deviation for the mean blank signal.
Hope this info will help you.
Good luck.
Grzegorz Boczkaj Thilini Madurangika Jayasinghe Karen A. Darbinyan
Thank you all for the information... but actually, I wanted to know the concentration range like in fM or pM that has been established as the limit for maximum fluorescence assays. I went through a lot of articles, but haven't been able to find any specific values like this. Most of the times there are methods given to calculate LoD as you have mentioned but I do not have a particular system in mind for which I'm trying to calculate. I just wanted to know the specs of the best performing fluorescence based systems.. so if anybody could direct me?
Thanks!
Hello Arti Tyagi , as said by Karen A. Darbinyan Fluorescence assays do not have normally defined LOD as well known analytical methods. there is no such generalization. Depending upon the fluorescent material u r using and the analyte u want to detect, the LOD will vary. It is better to search the literature for the analyte of your interest.
To the best of my knowledge, the best performing fluorescence based systems are having LOD in nM range (For some analytes it is uM only). There might be few systems with pM also.
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