Arti Tyagi

13 Questions 47 Answers 0 Followers

Questions related from Arti Tyagi

How does one calculate the magnification of a digital microscope on varying the distance of sensor from objective lens?

I have been struggling with this concept for a very long time. To calculate the total magnification provided by any digital microscope we generally multiply all the individual magnifications...

31 January 2023 4,170 6 View

Why am I not able to visualise milk somatic cells stained with FDA under fluorescence microscope?

I have been trying to label somatic cells isolated from milk (resuspended in PBS) using fluorescein diacetate (conc. of 8micrograms/mL) and view it under fluorescence microscope (Ex/Em used is...

26 August 2021 7,234 0 View

In terms of camera based fluorescence microscopy, what does time interval mean?

I am getting slightly confused here... When the term 'time interval or delta t' is used, does it explicitly imply time between successive frames or can it also refer to the time taken to capture...

20 October 2020 3,345 5 View

Does fluorescence based imaging improve spatial resolution?

I understand specific techniques based on fluorescence and other methods can enhance the spatial resolution beyond the diffraction limit of lambda/2NA (xy-plane) in what we call as the...

04 September 2020 9,031 3 View

Why doesn't the diffusion coefficient in the stokes Einstein eq. depend on the type of material?

I want to understand how can two spheres of different materials but the same hydrodynamic size and in the same solution have identical diffusion coefficients? Like shouldn't it also depend on the...

29 April 2020 9,675 16 View

How do I calculate the pixel size of my camera?

So as per my understanding the pixel size (let's say in microns) of a camera like ccd or scmos would also depend on the rest of the optical setup like the objective used. So one would carry out a...

12 April 2020 8,240 18 View

Which is the best cross linker for binding thiol-DNA to amine-magnetic beads?

I have to covalently conjugate thiolated ss-oligo probes (10-20 nucleotides long) on to the surface of amino-functionalised magnetic beads. I have been looking up papers on that and I came across...

09 January 2020 7,637 3 View

Why are zero mode waveguides made of aluminium?

All that I have read on ZMWs (not much) says "metallic subwavelength apertures" and then only mentions aluminium cladded ZMWs. Although I did find a comparison between Au vs Al (haven't read that...

24 December 2018 6,611 3 View

Is there any polymer I can use instead of poly(styrene/maleic anhydride)?

I am working on a project where I need to use the copolymer poly(styrene/maleic anhydride) and due to certain reasons I need it urgently. I cannot find anyone who'd deliver it so soon and so is...

02 November 2018 9,357 3 View

What is the difference between the various homologs of a single coenzyme?

I was looking for CoQ10 ELISA kits and I came across some labelled as 'CoQ10 binding protein homolog A, mitochondrial' or CoQ10... homolog B..' what is the difference between these two in terms...

16 April 2018 467 4 View

How can I prepare Candida cells for TEM analysis?

I found a protocol on the internet but it is a very elaborate one, is there a simpler one available? I need to visualise Candida cells alone as well as cells conjugated with MNPs.

02 April 2018 409 1 View

I was reading about the effects of Microwave heating on metal enhanced fluorescence based assays. In that context, I need to understand the following?

"For metals, the attenuation of microwave radiation arises from the creation of currents resulting from charge carriers being displaced by the electric field. These conductance electrons are...

29 November 2017 9,025 2 View

Fluorescence assays: limit of detection

what are the current achievable standards in terms of lower detection limit in fluorescence based assays?

01 January 1970 8,245 8 View