I did flowcytometry on the migrated t cells in chemotaxis assay to assess some surface markers. However the number of events is pretty low (less than 4000, aurora machine, high flow rate), which is expected because with routine protocols low number of cells would migrate in the chemotaxis assay. Do you have any suggestion to increase this number; or promote the flowcytometry technique or any other assay that I could replace by flowcytometry for measuring the surface markers?
After immunostaining the cells you can make a side to spin of the cells and then capture images of the cells and then use image analysis software such as metamorph to analyze cell associated immunofluorescence.
Try running triplicates. Then unite the migrating cells, and you MIGHT have enough for FACS
- Badges
- Science topic
- Similar topics
- Automotive Engineering
- Machines