Hello, fellow researchers! I am trying to develop a project to track the growth and development of embryonic and larval killifish (F. grandis and F. heteroclitus) in varying environmental conditions. Confocal or fluorescent microscopy are methods I am thinking of using, but I am unfamiliar with either of these techniques. I am hoping someone with more experience could help give me the pros/cons of both or provide resources to previous research using either technique so I can familiarize myself with what is possible and the methodology. Thank you for any help you can provide!
Hi Kayli Rose Jablonski , see similar approaches used in zebrafish imaging in recent work. Hope this might give you some insight.
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Sounds like lightsheet microscopy will be best. It provides fast and gentle fluorescence imaging, making it optimal for doing large volumes over long durations. https://www.nature.com/articles/s43586-021-00069-4
Kayli Rose Jablonski I suggest to read these articles:
1. https://labanimres.biomedcentral.com/counter/pdf/10.1186/s42826-020-00044-2.pdf
2. https://www.nature.com/articles/s41467-020-19827-1.pdf
3. Article Fluorescent Imaging of Cancer in Zebrafish
4. Article Highlights on selected microscopy techniques to study zebraf...
5. Article Watching the embryo: Evolution of the microscope for the stu...
6. https://protocolsmethods.springernature.com/posts/combined-whole-mount-fluorescent-in-situ-hybridization-and-antibody-staining-in-zebrafish-embryos-and-larvae
7. Article Identification and Characterization of Highly Fluorescent Pi...