Does anyone have idea about the photo-stability of FITC in nanoparticle formulations? Does it stable throughout the procedure and bright enough in confocal microscopy? Thanks.
We utilized FITC-avidin in our research for of ONBs but found out that they bleach out quickly. In fluorescence microscopy, the fluorescence was observable in the range of 10-20 minutes. I believe for confocal imaging, you need to cover them from light prior to imaging and then you can use it for imaging but the process needs to be quick.
Muhammad Saad Khan Thanks for the comment. Did you use any other alternative dye like Cy5 or Cy3 in your any other experiment? Thanks.
we have used Texas-red avidin conjugate in another set of experiments. It worked fine for fluorescence microscopy
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