How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Is there any software or formula for sample size determination by using sensitivity and specificity?

I want to conduct research on the diagnostic performance of some tests. I want to calculate sample size by using the sensitivity and specificity of the test in the previous studies. So Is there...

23 February 2021 619 5 View

What is the best way to track bird migration?

Migratory birds are globally scattered. What should be the ways to track them? Except ARGOS. Elaborate.

25 January 2021 2,355 4 View

How i can reduce the number of atoms using Vesta ?

I'm a new Vesta learner, I have downloaded cif of Ch3Nh3PbI3. Total number of atom in chemical formula for Ch3Nh3PbI3 is 12. But when i convert this cif to poscar file using Vesta, total number of...

14 January 2021 5,733 11 View

How to determine the sample size for ANOVA in GPower?

Hi, I am going to execute an experiment with the four categorical variables (A=2 levels, B=2 levels, C=2 levels, D= 5 levels) and one dependent continuous variable. There are total of 40 groups...

19 December 2020 3,600 4 View

How to calculate the replacement cost of battery?

As battery needs to be replaced after the specific charge and discharge cycles mentioned in the datasheet. If we have the time series data of battery power and energy capacity, then how can we...

01 December 2020 3,433 3 View

Can anyone please elaborate the relationship between charge and discharge cycles and battery lifetime?

I am designing the optimal HESS capacity for the renewable system. I have to select the cutoff frequency of low pass filter to divide the low and high-frequency components. charge and discharge...

05 November 2020 8,397 3 View

How to select the frequency range in MATLAB Wavelet toolbox?

I have a continuous, time-domain noisy signal and want to decompose into low-frequency (LF) and high-frequency(HF) components using Wavelet Toolbox. But, I am unable to select the frequency range....

29 October 2020 5,219 5 View

How can I retrieve information of viruses reported from a particular region/country ?

Dear All, Can anyone please guide me on how to get information (sequences) of plant/animal viruses from a specific region ? Looking up the published literature is one way but its time-consuming,...

19 October 2020 3,958 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View