Hello, I was wondering if anything is known about the concentration levels of fgf in glioma/GBM? I would be grateful for any information or paper suggestions!
Many thanks
I need advanced search like on Pubmed
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how do I create/copy a APA reference automatically from an article
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Dear researchers, I'm a master student in Belgium and I also work in psychiatric care for more then 12 years. I would like to recieve some tips or specific articles on exact numbers of...
16 March 2023 3,442 4 View
Dear all, I would like you to recommend me some papers that explore the impact of national culture on family firms, as it is a topic I would like to explore on my thesis. Thank you for your help.
02 March 2023 8,640 3 View
Hello. I have a problem with our in-house designed rt PCR for Cutibacterium acnes. Primers and probes were designed as part of mPCR. While testing each set of primers (monoplex) for LOD we are...
01 March 2023 4,356 1 View
Dear all, I am want to generate a k.o. with CRISPR/CAS9 in H292 cells and have issues with the transfections. It does not matte if its for plasmid or RNP format. Does anyone have a working...
02 September 2021 1,360 2 View
Dear all, we preparad samples with acetonitrile, nevertheless we have to storage them for some time. How can we avoid loss of acetonitrile in these samples? We tried storaging at -80°C,...
22 July 2021 5,273 2 View
A subset of my HL60 cells has red autofluorescence in the far red region (FL-3 > 670 nm). I first noticed this some months ago when I was checking an antibody with a PE-Cy5 tag by flow...
06 December 2020 6,189 2 View
Hello, I am planning a reserch which involves analysis of saliva cortisol in guinea pigs. Unfortunately, I am currently having trouble collecting saliva samples from my guinea pigs. I am not able...
30 September 2020 9,407 1 View
I am trying to transfect HFL-1 cells with a plasmid DNA. I used Lipofectamine 3000 from ThermoScientific, however the cells seem to react quite sensitive to the transfection (tried different...
17 November 2019 732 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Exism movements after gaining power within liberal democracies under majority rule and independent rule of law system become permanent dictatorship threats, but why this is the case is not clear...
04 August 2024 8,125 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How Social Media Affects Your Mental Health
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Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
I'm working on carboplatin which i bought from local pharmacy. It is a Carboplatin injection IP 150mg/15ml which is sterile aqueous solution for intravenous use. Unfortunately, I cannot able to...
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