What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

How to reduce background noise when testing cholesterol level in rat serum?

l've got rat serum samples with different background (from pink to yellow) and l'm trying to test total cholesterol, HDL and triglyceride in mice serum. l'm worried that the background noise...

14 November 2020 6,628 3 View

Is rotary evaporator a must before going through TPC, TFC and DPPH assay?

I am doing an optimization of ginger extract using doe software, firstly I extract the ground ginger with 20ml solvents of 60%, 70% and 80% ethanol using the ultrasound water bath in different...

23 October 2020 3,268 10 View

Failure in using Gene Pulser Xcell (Biorad) ?

02 October 2020 8,642 1 View

How to make the peak fitting better in TOPAS Rieltveld Refinement ?

Hi I am doing TOPAS Rieltveld Refinement by JEdit on PbTiO3-based perovskite solid solution found that my peak fitting is not that good. I got Rwp 4.584%, Rexp 0.825%, Rp 2.780, and GOF 5.560....

01 October 2020 1,981 5 View

How to quickly know the new research area landscape and find a research gap in this new area?

As a Ph.D. candidate who enters a new research area that differs from the research topic in the master period (e.g. shift form crop breeding to soil ecology), how to quickly know the new research...

23 September 2020 8,131 5 View

Is construction or real estate sector a pillar industry of the national economy?

Is construction or real estate sector a pillar industry of the national economy? The Chinese government once declared real estate sector as a pillar industry of the national economy in 2003. And...

02 July 2020 1,085 2 View

HEK-293t cell detach but still alive during killing curve set up?

Hi there, I'm trying to build a killing curve for HEK-293T towards hygromycin B for the following transfection experiment. I plated the cell at 1.3X10^5 cell/well in the 24 well plates the night...

30 June 2020 7,368 1 View

DNA absorbance test on SpectraMax Microplate Reader, why A260/A280 false?

Hi guys. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime,...

29 March 2020 5,972 3 View

What do chitosan-coated nanoparticles look like?

Hello! It is my first time to try to coat chitosan onto Fe3O4 nanoparticles. I am wondering what form of the chitosan-coated iron nanoparticles I should expect? After washing and drying, should...

28 January 2020 6,418 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

How reliable are degrees of freedom for lme mixed models in nmle package?

I am running mixed models analyses because I have participants nested in groups. Across the different analyses, I have very different degrees of freedom in the lme output with nlme package (and...

09 February 2021 6,767 3 View

Which software is the best to model a non-linear concrete (ABAQUS, ANSYS,ADINA, ATENA, etc.)?

What is the best finite element modeling software packages for modeling non-linear and fracture analysis of CONCRETE? I've had some with ABAQUS standart/explicit, but I would like to learn if...

27 January 2021 330 6 View

How plot results of membership function in R?

I am using the FRBS package, I developed the model without learning data and I use the plotMF function of this package to see the membership function of every input and output variables. I got the...

22 January 2021 2,668 3 View