Fasting time in db/db mice?

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Non incidence of diabetes in db/db mice ?

Whats the reason behind the diabetes incidence (blood glucose level less than 250 g% in db/db mice (of less than 50% of the animals ) in a db /db mice on B6 background even after 8 weeks of...

02 March 2018 8,960 2 View

Whether Cy 5.5 Dye pass BBB in mouse brain?

Whether Cy 5.5 dye pass BBB in mouse

08 September 2017 7,605 0 View

Does anyone have experience with beige mice genotyping?

I am developing a SCID beige double mutant mice and in F2 that I could be able to screen Prkdc gene for Scid and those positive for SCID needs to be screened for Beige mutation also. I am...

05 June 2014 8,295 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

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06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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05 August 2024 8,835 1 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

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05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

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How to report results of Generalised Linear Mixed Models in a journal article?

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04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

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In what part of the brain´s rabbit bdnf is located and include references please?

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04 August 2024 762 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Frédéric Gachon

If you want to perform a real fasting, an entire night is necessary before performing the GTT around 2 hrs after lights on

S.I.R. Okoduwa

A whole night fast is more appropriate. And the test should be conducted very early in the morning say between 6 and 8_am. If not you would face some metabolic effect on your results.