Hi everyone, I'd like to analyse mRNA expression level of gene in a treatment to wild type and analyse mRNA expression level gene between treatment in all group treatments.
I used T-test and there is no data significantly appeared. Then I tried F-test and the data become significant. Is anyone experience this? Thankyou
You might have a look at the attachment which may be useful to you. Best wishes David Booth 🙂
To get a reasonable answer, I think you'd need to clarify the question.
To me, it sounds like you have, say, 5 groups: say, 4 treatment groups and 1 wild type. You then used a t-test to compare 4 vs. 1 ? And then an analysis of variance to compare among the 4 treatments ?
Also, note that t tests and F tests can be used for all kinds of totally different purposes. Just saying "t test" isn't clear what you are doing at all. If I can try an analogy, it's like saying "I used a metal tool and a wooden tool". And we're like, "Used them for what? As a hammer? As a wrench? As a screwdriver?"
First, you should lighten your outline a little better. Do your groups follow a normal distribution? Then there are two groups or more groups? If you want to make comparisons with the control use HSD or Dunnett. You are comparing two tests that are for different types of oysters. If you can better clarify your goals and groups, maybe I can help you better.
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