use of ethyl acetate as a organic solvent has proven to give good product after extraction along with use of NaCl. Currently with the same system there has been enormous loss which one cannot understand, any thoughts on this
Sanket Rajendra Vakte
Emulsion Formation: Prevent emulsions by adding alcohol, centrifuging, or mixing gently. Volatile Compound Loss: Use a rotary evaporator at low temperatures under reduced pressure. Poor Phase Separation: Increase NaCl concentration to improve organic-aqueous separation. Inadequate pH: Adjust the pH to optimize metabolite partitioning into the organic phase. Low Solvent Affinity: Test other solvents or mixtures if ethyl acetate isn’t efficient. Incorrect Solvent Volume: Optimize the solvent-to-sample ratio for better extraction.
Loss during extraction with ethyl acetate and NaCl can result from:
These adjustments should reduce the loss and improve extraction efficiency.
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Abdelhak Maghchiche
Ethyl acetate is an effective solvent for extraction, but losses can occur due to incomplete extraction, emulsion formation, evaporation, or compound instability. Optimizing mixing, solvent volume, and extraction conditions is crucial for maximizing recovery.
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