Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

What is causing the raw fluorescence to be negative?

Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...

09 February 2021 4,642 3 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Can anyone suggest me broad host range fluorescence expression vectors for Gram-positive bacteria ?

Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...

06 February 2021 2,566 4 View

Are the hyperspectral image datasets which are publicly available at ehu.eus website atmospherically and geometrically correct?

I've come across a couple of publicly available hyperspectral image datasets from the website http://www.ehu.eus/ccwintco/index.php/Hyperspectral_Remote_Sensing_Scenes Now, my concern is...

05 February 2021 3,550 1 View

Any recommendations for open-source survey form builder, which has option for semantic differential scale questions apart from Likert scale?

Asking this as Google forms doesn't allow multiple items in a semantic differential format.

04 February 2021 5,869 4 View

What are some in vitro experiments that can be performed to target hypoxia?

I want to show that my developed nanoparticle targets hypoxia but from doing research it seems like most hypoxic targeting related experiments are done in vivo. I'd like to be doing in-vitro...

16 August 2020 3,695 4 View

How long a hydrolysate for hydroxyproline can be stored at room temperature for Hydroxy proline assay?

We use 12N Hcl (20 volumes) to digest the tissue at 110-degree celsius for overnight, to do colorimetric assay for hydroxyproline assay. The hydrolysate will be centrifuged and supernatant will be...

29 May 2020 7,119 1 View

What is the best stock concentration and final concentration of Cobalt 2 chloride hexahydrate?

Hello Everyone, I have some questions related to a drug called Cobalt 2 chloride hexahydrate. I am currently preparing an experiment related to Hypoxia. I bought a drug called " Cobalt 2...

10 May 2020 4,841 4 View

If Proline and Glycine are incompatible with helical structural conformation of protein, why do these amino acids are abundantly found in Collagen ?

It's said that Proline and Glycine amino acids are incompatible with helical structure and form kink in the helix, but why do Proline and Glycine are abundantly found in the collagen ?

08 September 2019 9,500 4 View

Why is my Proline-ninhydrin reaction test turning purple?

I am using the ninhydrin method as a way to quantify the amount of proline in a particular sample. I've started off testing out my method using pure L-proline as a test. However, the reaction...

21 May 2019 6,424 8 View

How can I make drop out media plates containing Kanamycin or Nat drugs for yeast ?

I found out that Nat or Kan selection does not work on drop out plates. The ammonium sulfate interferes with drug selection. I have read that using proline or glycine instead of ammonium sulfate...

01 February 2019 2,348 3 View

Will my product recrystallize?

Hello, So I would in a small research Lab doing undergraduate work and we are using the base unit of proline with different functional groups on it. Mine specifically has a TBDMS protected...

27 December 2018 3,581 3 View

Why metal, acids and amines catalysts are low reactive than l-proline?

NA

11 December 2018 117 1 View

What are the extraction and dosing techniques for bacterial osmoprotectants?

Greetings, I'm searching for methods to extract and dosing some of bacterial osmoprotectant such as ectoin and betaine glycine and proline from bacteria Thank you

11 December 2018 7,824 0 View

No title

10 November 2018 498 0 View