Experience with IC50 plotting?

02 February 2016 0 4K Report

pls share your experience on generally how many conc to activity points did it take to generate a graph for IC50 estimation?

Badges
Science topic

Similar topics
Mathematical Sciences
Graphs

More Sabyata Pharmacy's questions See All

Is a drug always more soluble than its hydrate counterpart?

I am buying some compounds for enzyme inhibtion assay. I want to use neutral compound and not salt form. I don't clearly understand if there is diff in assay with neutral or salt form but for now...

02 March 2016 4,200 4 View

Help with protein inhibition assay setup?

i want to DPP4 protein inhibition assay of at least 10 compound with 96 well plates. Both the standard drug (sitagliptin) and the substrate is present at 100uM conc during assay. I want triplicate...

01 February 2016 1,507 7 View

Can the same micro plate reader used for ELISA testing be used for enzyme inhibition assay that reads colorimetric absorbance?

I am confused- there is both colorimetric and flurorimetric ELISA which is done in micro plate reader. I am screening enzyme inhibitors and the product says colorimetric assay but it is not a...

01 February 2016 6,522 4 View

Pubmed search help?

i need to check if a compound has been tested for a certain protein. Pubmed seems to have such info but there is a ton of things and very confusing i need two straight process 1) A list of all the...

10 November 2015 853 2 View

How can I calculate IC50 in DPPH test by using graph pad prism software?

How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.

06 August 2024 8,848 3 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

What is meant by baseline of FTIR data?

I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...

03 August 2024 6,070 3 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

Entropy measure and QSPR modeling in Graph Theor. How to construct the table for lengthy equation?

The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...

30 July 2024 3,126 0 View

There is possible way to calculate experimental DOS from the UPS spectrum of the material. How to calculate the DOS from UPS?

Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.

29 July 2024 4,971 1 View

Critique Calculaltion in Topological Indices and QSPR Modeling?

The first step is to analyze a 2D molecular graph and implement partitioning techniques to calculate the topological indices. Secondly impose statically tools to generate QSPR model, may or may...

24 July 2024 9,644 1 View

How to do calculation of Louvain methods calculation for excel sheet and and is there any software available?

The Louvain method – named after the University of Louvain where Blondel et al. developed the algorithm – finds communities by optimizing modularity locally for every node’s neighborhood, then...

23 July 2024 6,659 0 View

Is there a name for this type of function?

Let n∈ℕ, where the function f:A⊆ℝ𝑛→ℝ, 𝐴 is Borel, and (A,d) is a completely seperable space. What is the name of the functions where the graph of f in any (n+1)-dimensional box of A×ℝ is...

17 July 2024 258 1 View

Why is linearity somewhat lost when plotting the Stern-Volmer (SV) graph?

When preparing a graph of fluorophore intensity vs. analyte concentration, the linearity shows an R² of 0.999. However, when plotting the same data as Fo/F vs. analyte concentration, the linearity...

13 July 2024 7,131 0 View