Sabyata Pharmacy

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Questions related from Sabyata Pharmacy

Is a drug always more soluble than its hydrate counterpart?

I am buying some compounds for enzyme inhibtion assay. I want to use neutral compound and not salt form. I don't clearly understand if there is diff in assay with neutral or salt form but for now...

03 March 2016 4,182 4 View

Help with protein inhibition assay setup?

i want to DPP4 protein inhibition assay of at least 10 compound with 96 well plates. Both the standard drug (sitagliptin) and the substrate is present at 100uM conc during assay. I want triplicate...

02 February 2016 1,486 7 View

Experience with IC50 plotting?

pls share your experience on generally how many conc to activity points did it take to generate a graph for IC50 estimation?

02 February 2016 4,090 0 View

Can the same micro plate reader used for ELISA testing be used for enzyme inhibition assay that reads colorimetric absorbance?

I am confused- there is both colorimetric and flurorimetric ELISA which is done in micro plate reader. I am screening enzyme inhibitors and the product says colorimetric assay but it is not a...

02 February 2016 6,503 4 View

Pubmed search help?

i need to check if a compound has been tested for a certain protein. Pubmed seems to have such info but there is a ton of things and very confusing i need two straight process 1) A list of all the...

11 November 2015 834 2 View