Hello everyone,
Is it possible to calculate the E/I ratio on single-cell levels only based on miniature signals (Excitatory and inhibitory)?
If yes, then what parameters should I take to calculate it? and how?
Currently, I can get lots of parameters from mIPSPs and mEPSPs such as the number of events, decay and rise time, area, baseline, noise, half-width, 10-90 slope, etc...
Which one of these can i use to calculate the E/I balance and the calculation mathematical formula?
Thank you!
Hello,
If it can help you, the calculation for EI decomposition has been well described in these publications :
Le Roux, N., Amar, M., Baux, G., and Fossier, P. (2006) Homeostatic
control of the excitation-inhibition balance in cortical layer 5 pyramidal
neurons. Eur. J. Neurosci. 24, 3507–3518
Lucas-Meunier, E., Monier, C., Amar, M., Baux, G., Fr´egnac, Y., and
Fossier, P. (2009) Involvement of nicotinic and muscarinic receptors
in the endogenous cholinergic modulation of the balance between
excitation and inhibition in the young rat visual cortex. Cereb. Cortex
19, 2411–2427
Moreau, A. W., Amar, M., Le Roux, N., Morel, N., and Fossier, P.
(2010) Serotoninergic fine-tuning of the excitation-inhibition balance
in rat visual cortical networks. Cereb. Cortex 20, 456–467
Moreau, A. W., Amar, M., Callebert, J., and Fossier, P. (2013)
Serotonergic modulation of LTP at excitatory and inhibitory synapses
in the developing rat visual cortex. Neuroscience 238, 148–158
Meunier,C.N. J.,Amar,M.,Lanfumey, L.,Hamon,M., and Fossier,P.
(2013) 5-HT(1A) receptors direct the orientation of plasticity in
layer 5 pyramidal neurons of the mouse prefrontal cortex.
Neuropharmacology 71, 37–45
Meunier, C.N. J., Cancela, J.-M., and Fossier, P. (2017)Lack ofGSK3b
activation and modulation of synaptic plasticity by dopamine in 5-
HT1A-receptor KO mice. Neuropharmacology 113, 124–136
Best regards,
Lora
Hello,
I think you can calculate E/I ratio by using mEPSC and mIPSC amplitude. For example, firstly detect the mEPSC amplitude and mIPSC amplitude at the -70 mV (or at the another holding potential). Then, take the ratio of these values, you would calculate the E/I.
What do you think the E/I ratio is? What do you hope to show? If you wanted to, you could calculate the total charge transfer due to mIPSCs, and the total charge transfer due to mEPSCs, and take the ratio of those, but what do you think that would demonstrate? Do you think that metric would have the same information as when someone reports the time varying E/I balance due to a sensory input? Think about what the rates of minis represent, think about what the amplitudes represent. And think about whether if you did some math on these, whether they would answer the question you hope to answer.
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