Is there a method for estimating the OD of an E. coli culture without using the spectrophotometer? [I need to estimate an OD600 = 0,4 - 0,8 for electrocompetent e coli]
Note that you still need some apparatus such as a colorimeter or other device if you want accuracy and reproducibility, although you can do it by eye (if you have the standards) if you are satisfied with approximate. You can also count cells per volume microscopically (after the right dilution of course) using a counting chamber. w