What colour is a resorufin solution at pH < 6.1?

The redox dye resazurin (dark blue) can be reduced in aqueous solution at pH > 6.1 via resorufin (pink) to dihydroresorufin (colourless). What colour is the resorufin solution at pH < 6.1?

21 January 2021 8,442 2 View

We use Thermo Scientific Superfrost plus gold coated slides. Why our cryo sections of dry fruits do not stick to the slides?

We use Thermo Scientific Superfrost plus gold coated slides. Why our cryo sections of dry fruits do not stick to the slides?

13 January 2020 1,215 1 View

My mysterious red dye may not be neutralized before HPLC/MS as it is only stable between pH 12.5-12.9. Who could help with equipment tolerating pH13?

More information in https://www.chemistryviews.org/details/education/10821368/150_Years_Alfred_Wohlk.html

04 July 2019 1,409 1 View

Are smaller insect species more sensitive to residual insectides?

Alarming declines of insects elicited a vivid public debate, particularly in Germany (#Insektensterben), but also in many other countries (see...

06 September 2017 7,975 4 View

Someone knows a company that offers gene synthesis in expression plasmids for use in plants?

16 May 2016 8,212 3 View

Which flower signals are honest?

There are some reports about honest floral signals, e.g. flower colour change in many flowering plants correleted to nectar production, size of Dalechampia bracts indicating the size of a resin...

25 April 2016 1,418 5 View

Which pan trap colors are attractive for bees?

Dear All, I want to learn about pan trapping of bees and the most attractive colours. Does anybody know published spectral reflectance curves of pan trap colors? Can anybody explain the term...

08 March 2016 7,710 44 View

How does flower suntracking correspond to bee foraging behaviour?

Many flowers show heliotropism and the flowers are sun tracking. The discussed benefits of sun tracking flowers are heating up the flowers for better pollen germination and seed production, and...

26 January 2016 9,316 5 View

Does it make sense to compare linear regression slopes in the Murmansk region (Russia) using air temperatures from gridded E-OBS data?

12 June 2015 8,483 14 View

Why do some researchers neglect multiplicity in the calculation of statistical significance?

14 February 2014 786 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Hi all, My research questionnaire has 3 sections each having a mix of yes/no and multiple choice questions. How can I test its reliability ?

The sections are about awareness, practice, and perception regarding waste management. Thanks

20 February 2021 5,722 3 View

How to perform Kaplan-meier survival analysis in SPSS?

Hi everyone, This is my first time of attempting to run survival analysis. I have a data on patients with End-stage Kidney Diseases (ESKD). I would want to run survival probability by treatment...

16 February 2021 2,647 3 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

How to inter pain intensity data using NRS when patients report their pain in spectrum?

I have been working on a pain intensity study and I wanted my patients to report their pain intensity using numerical rating scale. many patients have reported thier pain in spectrum like 2 to 3...

13 February 2021 6,598 3 View

What is the best way to calculate sample size for a multivariate regression model for adequate power?

GPower is only equipped to deal with calculations of bivariate regressions and needs SD s for both predictor samples. However, if one has a multiple regression with more than 2 outcome variables...

12 February 2021 6,911 3 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View