Hello,
I am looking for an efficient method to separate My Enzyme from my reaction mixture. I tried filtration many times but I am Never able to fully separate the enzyme from the reactants. If I just filter it, the enzyme Looks more like powder or chunks than how it was originally used. Any advice how to improve the purification of the enzyme?
I added a picture With the enzyme and also the left moleculare sieves.
More information is needed to answer your question.
Please describe your starting material, including as much of the following as you know or can determine:
- Volume percent solids
- Particle size distribution
- Physical nature of solids (shape? compressible or rigid?)
- Liquid viscosity
- Density difference between liquid and solids
How are you filtering now? Vacuum filtration using a Buchner funnel? What size and type of paper? Please describe the results in as much detail as possible (for example, most solids are retained and only fines go through?).
How does the molecular sieve fit into your process? Does it need to be filtered out as well? If yes, please provide as much of the info in the above bullets as you know.
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- Science topic
- Similar topics
- Control Systems Engineering
- Filtering