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Why do you use a coating buffer containing carbonate / b-carbonate for ELISA? Do you use a combination of carbonate and bi-carbonate? Or do you just use one of them? If yes, when do you use...
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Hello, I am looking for an efficient method to separate My Enzyme from my reaction mixture. I tried filtration many times but I am Never able to fully separate the enzyme from the reactants. If I...
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Hello, I need help to purify my product benzyl butyrate from my lipase-katalyzed esterification. I am using Novozym ® 435 and in addition molecular sieves. I want to quantify the amount of...
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Hi, I need help to find a fitting method to determine the esterification activity of a lipase. I am using the Novozym 435 and I am also using a very small volume in this reaction. The enzyme...
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