5 Questions 3 Answers 0 Followers
Questions related from Sarah Mattern
Hello, I have a question regarding PCR. I am trying to do a site-directed mutagenesis on my plasmid. I use fresh PCR water, change tips after adding something into one tube and I also let all...
21 April 2023 7,742 4 View
Why do you use a coating buffer containing carbonate / b-carbonate for ELISA? Do you use a combination of carbonate and bi-carbonate? Or do you just use one of them? If yes, when do you use...
06 March 2023 3,248 5 View
Hello, I am looking for an efficient method to separate My Enzyme from my reaction mixture. I tried filtration many times but I am Never able to fully separate the enzyme from the reactants. If I...
08 December 2020 8,948 2 View
Hello, I need help to purify my product benzyl butyrate from my lipase-katalyzed esterification. I am using Novozym ® 435 and in addition molecular sieves. I want to quantify the amount of...
13 October 2020 2,272 3 View
Hi, I need help to find a fitting method to determine the esterification activity of a lipase. I am using the Novozym 435 and I am also using a very small volume in this reaction. The enzyme...
23 September 2020 5,986 6 View
