How to compute correctly the heat flux in order to obtain a stable thermal/thermo-hydraulic coupled simulation ?

Dear all, I am trying to couple a simulation with two codes, say code A and code B (different discretizations). For code A we solve simply the conduction equation (solid), while in B we solve the...

24 November 2020 8,961 2 View

How do I run a western blot following co-ip?

This is the first time I do any sort of IP experiment and I was wondering if there is a certain procedure to follow for the western blot after the last elution step in the co-ip protocol. I am...

22 September 2020 3,688 3 View

How to properly normalize my absolutely quantified mRNA expression?

My PI wanted to use a standard curve to absolutely quantify mRNA expression via RT-qPCR. Her idea was to create the standard curve from a stimulated cell line that expressed the gene of interest...

19 September 2018 4,368 16 View

Can someone help with standard curve for competitive ELISA?

I used a competitive ELISA to look for collagen IV. I got the standard curve numbers. The instructions in the manual state that the data may be linearized by plotting the log of concentrations...

22 March 2018 6,633 2 View

Is there a specific method to set a Mach Zehnder interferometer for surface relief grating inscription on polymer thin films?

Hello, I have built a Mach-Zhender interferomer in order to irradiate thin films of polymer (PMMA) with an interference pattern. These polymer thin films are doped with metallic precursors, and...

25 May 2016 4,245 4 View

What initiator should I use to prepare a nanocomposite made of Cobalt nanoparticles dispersed in a PMMA matrix?

29 February 2016 6,294 1 View

Calculating the effective mass manually

I am trying to calculate the effective mass of electrons and holes manually from the band structure of a 2D system I am working on. I have only considered the maximum valence band and the minimum...

01 January 1970 4,055 3 View

How to compute correctly the heat flux in order to obtain a stable thermal/thermo-hydraulic coupled simulation ?

Dear all, I am trying to couple a simulation with two codes, say code A and code B (different discretizations). For code A we solve simply the conduction equation (solid), while in B we solve...

01 January 1970 9,673 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View