I have some worries about the observation of blades in cytology.
These blades are produced from tonsil samples in ThinPrep and are very rich in lymphocytes. One does not wish to solely have epithelial cells.
I would like to eliminate most of the lymphocytes before observation.
One does not wish to use FACS for cost reasons.
Would you have any idea about the separation of the cells by density gradient, like the Ficoll technique for the PBMC?
Namely because ThinPrep contains liquid of conservation (methanol 50%).
P.S. some examples of observation
Hi Babak,
Sorry about delay too.
I see your techniq wich use nylon wool, but this techniq is efficient for purification of lymphocyte. But here my question is the reverse, i wish purificate my epithelial cell and eliminate lymphocyte.
Thank you Babak.
Alexandre.
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