Hello everyone. I am recently focusing on dendritic spine dynamics in pain experiment. I wonder are there big different effects in visualizing dendritic spines between using EGFP and EGFPf (membrane-targeting GFP) or not?
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View
To generate curve but a straight line generated on zero load.
09 July 2024 2,617 1 View
Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...
01 July 2024 8,964 1 View
I'm trying to stain areas of dystrophic calcification in sections of FFPE perinatal brain tissue. Typical basophilic appearance on H&E but nothing with Alizarin Red (costochondral junction...
28 May 2024 4,068 2 View
Why we checking the GCA of a parent during hybridization program?
26 May 2024 590 1 View
at the time of filling the peroxide value is 0.5 however it increases with the passage of time. we have never faced such problem in past. please someone assist me in this regard.
22 May 2024 6,296 2 View
We have two sets of data, and we want to conduct SEM-PLS and multi-group analysis via SmartPls. However, one set of data is 5 point Likert scale while another is 7 point Likert scale. Do I need to...
21 May 2024 8,548 4 View
what's different between soil nitrogen pool and soil phosphorus pool?
18 May 2024 5,677 2 View
Hello, I am new to mixed effects model and am running a analysis with it. However, am wondering if the assumptions check for Quadratic mixed models are the same as for linear mixed model? -...
15 May 2024 7,716 3 View
I would be extremely grateful if someone could help me find the following cards: CaZnOS PDF-01-072-3547 SrZnOS PDF card # 041-0551 BaZnOS PDF #17-1239 Ba0.5Na0.5TiO3 - SrTiO3 Thanks a lot!
08 May 2024 5,034 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View