Efficient method to isolate peptides from Ascidian?

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What is the optimum percentage of SDS - PAGE gel for screening unknown peptides ???

We need to isolate peptides from Marine animals...So can you all suggest the optimum percentage of SDS - PAGE gel

01 February 2017 3,515 8 View

Can anyone suggest the best inducer to induce Alzheimers in a zebrafish model?

i need a list of inducer that can Alzheimer like symptoms....other than heavy metals and stress inducer???

09 October 2015 2,195 2 View

What are all the biochemical and molecular techniques for to identify in human blood &serum samples Neuroinflammatory affected patients?

Please refer me some articles and techniques to carry out this research... I think to include this in my research

08 September 2014 4,130 1 View

What is the best method to induce neuroinflammation in rats and mice?

see above

02 March 2014 442 2 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

What strains of C57 mice can induce rheumatoid arthritis?

Recently I looked up a literature《A Glucose-6-Phosphate Isomerase Peptide Induces T and BCell-Dependent Chronic Arthritis in C57BL/10 Mice Arthritis without Reactive Oxygen Species and...

09 July 2024 6,657 0 View

I found trouble to run MD of my peptide with PTM in different force field. In Charm-GUI, it cant recognize the engineering residue. What can I do now?

I am working on my PTM project. I have some peptide. I got the result of doing MD of those peptide without PTM in different force field like Charm-GUI, Amber, Gromacs. Now I have to do MD of those...

05 July 2024 9,346 0 View

How to prepare a transparent solution of the peptide with the mixture of hydrophobic and hydrophilic amino acids ?

I am using a peptide and have faced challenges in preparing a transparent peptide solution. My goal is to prepare a peptide solution in MES buffer and then use the solution to immobilize the...

20 June 2024 8,622 5 View

How do I calculate endogenous peptide using Isotope Dilution Mass Spectrometry (IDMS)?

Dear Researchers, I'm trying to develop the quantification of endogenous peptide in tissue using IDMS as the primary method of characterization. I performed analysis for the calibration curve of...

17 June 2024 9,797 0 View

Alexander Mikhailovich Zakharenko

Hi, Manigandan Venkatesan,

you can try to make phosphate or tris buffer extraction with followed by addition of acetone to precipitate high molecular weight proteins and polysaccharides

Manigandan Venkatesan

Dear Sir

Thank you..what about the solvent based extraction methods for peptides...Is that not suitable for peptide extraction...