How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Are most sol-gel adhesion issues because of surface impurities?

We are trying to get a sol-gel film coating on diecast aluminum/silica (87%/13%) parts, and it is flaking off quite rapidly. This same flaking isn't happening with prototype milled aluminum...

13 January 2021 1,110 4 View

What are the chemicals that can prevent the self-healing concrete to happen?

I know the pH has a big impact on the bacteria and there are some types of bacteria that can stand the high alkali. However, I just want to know what are the other factors that prevent...

02 November 2020 7,025 5 View

Why did i not getting plasmid band in electrophoresis even when i got pellet after alkali lysis?

Is there any reason for i failed getting any bands on electrophoresis after i did plasmid isolation from E. coli DH5a? My plasmid is ~17 kb in size and i already checked the transformant with...

16 October 2020 6,832 5 View

How to determine the moles of alkali required for saponification of the oil for fatty acid extraction?

I have calculated the saponification value of the oil. Now, what is the calculation to determine the amount of alkali required for the saponification value? How to determine the molecular mass of...

20 September 2020 3,492 3 View

Conversion of Dichloroalkane into Alkanediols?

I need to convert 1,2-dichloralkane to alaknediol using reflux condenser. The dichloroalkane is insoluble in water. Hence i need to take methanol/ethanol as solvent. For substitution reaction i...

14 September 2020 6,292 3 View

Is that possible the occurrence of biotite, hornblende, sphene and apatite in the same granite and which type of geological setup is indicate?

My study area is restricted to the central part of Singhbhum Craton surrounding by Iron Ore Group (IOG) belt in the Eastern India, India. In the mapped area meta-volcanic and meta-sedimentary...

14 March 2020 2,801 3 View

What are the most applied solvents to help epoxy resin to penetrate through granite stone?

I'm looking for a solvent that could penetrate epoxy resin (mw~400) into the granite stone

06 March 2020 420 2 View

When coal fired power plants are eliminated, what will be the source of fly ash for concrete?

Fly ash is used in concrete to help eliminate the alkali - silica reactivity between portland cement and the surface of the aggregate. Alkali - Silica reactivity can turn concrete highways into...

27 January 2020 6,027 3 View

When coal fired power plants are eliminated, what will be the source of fly ash for concrete?

Fly ash is used in concrete to help eliminate the alkali - silica reactivity between portland cement and the surface of the aggregate. Alkali - Silica reactivity can turn concrete highways into...

31 December 2019 5,455 3 View