Effective way to break open and sterilise Bacteroidetes cells?

Katrin Leinweber @Katrin_Leinweber

12 December 2013 1 1K Report

I want to extract intracellular material from a Bacteroidetes strain, but I can't use temperatures above 60°C.

Does anyone have experience in the effectiveness of ultrasound, lysozyme or other methods?

Katrin Leinweber

Note to self:

- fill up Bacteroidetes suspension with EtOH to a concentration of 70% EtOH

- let shake in sterile bench at 60°C until EtOH evaporated (20min for 100µL in my case)

- repeat once & let dry completely (1h for 100µL in my case)

- resuspend yellow-white-ish residue (in same medium as the suspension had) and test for sterility in LB (no growth in my case)

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