Hi All,

My insert is cloned into pcDNA3.1. The full insert sequence shows the surrounding vector sequences including plasmid promoter and terminator are clean and tidy. The 3’UTR of the gene of interest is also present in the insert.

My question is that will COS cells recognise the transcription termination signal in the insert and will this effect the expression of my gene of interest using the pcDNA3.1 vector. Will the inclusion of 3’UTR have a possible effect on the efficiency of termination??

Hope to hear from you soon.

Thank you :)