E.coli, pink colonies.

19 March 2013 0 1K Report

We are having a contamination on our LB plates. Besides E.coli colonies, we have some pink colonies. Could they be lactobacillus or they are E.coli that turn pink?

Badges
Science topic

More Lucila Giordana's questions See All

Does any one have research on the impact of age and gender in Simultaneous interpretation quality?

I am a PhD student looking for information on research carried out recently linked to qualtiy in SI specially around age and gender. Lucila Christen

14 September 2021 2,738 0 View

How can I work with statistical programs if I have abundance data for all species and biomass data for one of them?

Hi, this is my first question on research gate, so I hope I am doing this right. I have to work with PRIMER-e software with a lot of data from zooplankton species. All data was collected as...

12 November 2020 7,501 3 View

What is the best plasmid for Bacillus thuriengiensis transformation (by electroporation)?

I am trying to overexpress proteins in Bt. I would like to transform Bt by electroporation. I have protocols to do that but I am not sure about the plasmid characteristics

08 July 2017 1,576 2 View

How is birth order related to leadership?

How does birth order impact the leadership style?

24 March 2016 341 5 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Are my cells contaminated with mycoplasma?

I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...

11 July 2024 7,786 3 View

Black dots like contamination in my cell cultures. What are they?

There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...

11 July 2024 4,829 0 View

Does the soil contamination correction factor for decomposing leaf biomass vary according to space and season?

The question refer to leafy biomass decomposition using litterbag techniques.

11 July 2024 2,078 0 View

When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?

07 July 2024 5,480 2 View

What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?

02 July 2024 9,204 0 View

Why can't we get on top of this incubator infection (fungal)?

We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...

02 July 2024 6,375 2 View