Does anyone know a way of differentiating E14 stem cell into cardiomyocytes without using hanging drop method? Easy 6 well culturing and harvesting for biochemical assays.
Hi, I did the differentiation of E14tg2a cells via embryoid body formation on low adherence plates/wells in absence of Lif. Within one or two weeks some beating bodies will form which can be plated or harvested for analysis.