during purifying e coli clpB from digested sample, it is all getting lost somewhere in between step. what can be the possible reasons... i have done this experiment thrice with all possible ways to reduce error by my knowledge...
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Hi everyone, Does anyone know how Frequency Selective Scheduling is implemented in a 5G Network? A step-by-step understanding would be appreciated. Any references or articles will be good. Thanks.
30 July 2024 4,963 2 View
Any leads with proper explanation would be helpful.
02 July 2024 5,649 2 View
In my case , as i increase the current density the specific capacitance of the electrode is increasing. Also, I am getting the least capacitance in case of 1 A/g and maximum at 10 A/g. Can anyone...
19 May 2024 1,422 0 View
I have been trying various protocols, I was successful at isolating cells from tissue via both explant and enzymatic method, however after some days the cells are becoming stagnant and are not...
14 May 2024 8,846 0 View
Can anyone please
30 April 2024 1,444 0 View
I have taken fluorescence images of the control and treated sample(Immunofluorescence, tissue sample) at the same settings. So I need to measure the change in fluorescence intensity of the treated...
16 April 2024 2,093 1 View
I am working on a deep learning model with following GAN(Generative Adversarial Network) architecture and got stucked on dataset creation(using tensorflow function to simplify the data for my...
05 April 2024 6,628 1 View
currently, i am working on cancer cells and trying to study the effects in combination. One is a known anticancer drug but the other does not show any anticancer properties however it can reduce...
17 March 2024 9,764 1 View
Hello everyone! I am preparing electrospun fiber of PLA in Chloroform/DMF solvent (9:1 ratio)...I am following standard reported protocol but i am not getting fiber deposition on collector much....
15 March 2024 4,608 2 View
I am inclined to research on EEG classification using ML/DL. The research area seems saturated. Hence, I am confused as to where I can contribute.
14 March 2024 7,060 1 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...
22 July 2024 412 3 View
One has to understand about the enhancement procedures and treatment systems I can list around 12 procedures which are tried and tested and are proven so let us see how many in total the experts...
20 July 2024 3,392 2 View
Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...
18 July 2024 5,225 3 View