during purifying e coli clpB from digested sample, it is all getting lost somewhere in between step. what can be the possible reasons... i have done this experiment thrice with all possible ways to reduce error by my knowledge...
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Why thermally evaporated (VCM 600 V1 configuration of the thermal evaporator) CdTe film found n-type not p-type conductivity whereas all CdTe films deposited by varying the source to substrate...
23 November 2020 7,427 3 View
Hi, I have Fold expression of RNAseq data obtained from Ion Proton sequencer and got the fold change data with mean in an excel sheet. now can I analyse this fold change data using any...
20 October 2020 2,240 3 View
Concrete column casting after masonary completed monolithic.
17 August 2020 7,172 3 View
If temperature reinforcement already provided ,at reentraint corner need extra diagonal reinforcement ,if needed then what amount of steel.
12 August 2020 1,707 2 View
26 July 2020 1,511 0 View
I am doing a blood flow research for which Time average data of Velocity and Wall shear stress is important. The study is transient and my flow time is 0.5 sec. I save data with time step size of...
07 July 2020 4,323 2 View
What should be the best managemnt option for suppressing the DBM problem that farmer can take into consideration?
11 May 2020 4,280 1 View
I had submitted a research article related to the field of mechanical engineering (CFD) on the last December, 2019 in a Scopus Indexed journal. It has been around 6 months but the journal...
11 May 2020 9,286 8 View
Basically I need the CIF FILES for doped system. If someone have CIF for at least doped composition in any allowable stoichiometry, could it be shared. I am doing a simulation and for that I need CIF.
06 May 2020 6,239 2 View
I have been working on peptide vaccine for SARS-CoV-2 or COVID-19 and also recently published some analysis on mutational changes in this virus. It would have been really helpful if I could get...
05 May 2020 2,749 9 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...
27 February 2021 947 7 View
Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?
26 February 2021 3,487 3 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?
23 February 2021 3,053 7 View