PCR used to work, now it doesn't, why?

I recently did a PCR with certain primers, and the reaction went well for two gels, but when I repeated the exact same reaction a third time it suddenly didnt work, then I did it a 4th time and it...

12 December 2020 364 5 View

How to model bubble in Ansys Fluent?

I am seeking to model a simple bubble growth/collapse in Ansys. I have tried using 2D, VOF with an initial patch air circle geometry surrounded by water. I include surface tension and make the air...

10 December 2020 9,113 3 View

DNA disappears from gel, whats going on?

Recently I've been doing some PCRs and the primes used to work a month ago, but now that we want to repeat the experiment, they are not working anymore. I have noticed something in my gels, and it...

28 October 2020 4,427 4 View

How to cite chinese names?

This may look like an odd question, but since I'm latinamerican I'm not familiar with chinese names, the usual way to reference names is by writting the last name and the initial of the names, but...

11 September 2019 9,386 3 View

ANOVA with 4 factors to contrast seasonal curves?

28 April 2019 5,115 1 View

ANOVA with 4 factors to contrast seasonal curves?

03 April 2019 4,284 1 View

Does anyone knows this plant?

I need samples of a plant named Kermadecia sinuata, according to the data I got it is found in New Caledonia, anyone knows any repository of exotic species that could have this specimen and that...

12 February 2019 2,125 2 View

How to separate very closed PCR bands?

I've been trying to purifiy my electrophoresis bands but they are very closed to each other, I've tried 2% agarose gels, ran at 90V for 2 hours and this is as far as I can get them to separate. My...

07 November 2018 5,383 3 View

Somebody has isolated DNA with basic phenol using the DNA/Protein that is precipitate using the protocol of RNAzol or Trizol?

I perform total RNA isolation according to RNAzol RT protocol from bone marrow samples, but I need the DNA of this samples too. Some protocols explain that I can use DNAzol to isolate the DNA but...

03 April 2018 3,979 4 View

Is there any research about traditional cheese-pressing?

We are analysing the traditional ways of cheese-pressing in the Basque Country. There are many materials about cheese and cheese-making, but, unfortunately, we have not found anything about...

26 February 2018 5,870 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View