Doubled haploid barley production?

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Ewa Nowak

Thank you Sezer.

María Muñoz-Amatriaín

I have used 0.7 M mannitol followed by FHG medium + Ficoll 400 in the laboratory of Luis Cistue, and it worked very well (except for some genotypes generating a high percentage of albino plants).

The detailed method is published in the book 'Doubled Haploid Production in Crop Plants' (2003), pp 29-34.

Thank you Maria.