Dose anyone know why pCAMBIA2300 didnot give any clonoy when cloned into DH5a ?

12 December 2018 0 7K Report

I digested my plasmid pCAMBIA 2300 and fragmrnts using BamHI and SacI enzyme then, I pricipatae the sample using EtOH, then ligate them using the T4DNA, then I try to colne my plasmid backe to E.Coli (DH5a), I didnot get any colony. although my fragments and plasmid in a good concentration > 5ng/ul, and the positive control gave some coloies.

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