Mona Albloushi

4 Questions 13 Answers 0 Followers

Questions related from Mona Albloushi

How to transfere fragments into pCAMBIA 2300?

I had amplified my fragments with primers to give an extra base pair for the restriction site, which has been digested. furthermore, the vector has been digested with same enzyme SacI. but my...

01 January 2019 7,066 3 View

Dose any one know if Iwant to digest pCAMBIA plasmid using double digest how can I do it?

Trying to digest the pCAMBIA plasimd using BamHI and SacI which buffer should I use Is the Tangu buffer will work for both at the same reaction. my protocol is: 3ul Of each enzyme, 3ul of the...

12 December 2018 1,724 13 View

Dose anyone know why pCAMBIA2300 didnot give any clonoy when cloned into DH5a ?

I digested my plasmid pCAMBIA 2300 and fragmrnts using BamHI and SacI enzyme then, I pricipatae the sample using EtOH, then ligate them using the T4DNA, then I try to colne my plasmid backe to...

12 December 2018 6,746 0 View

I try to Digest the pCAMBIA 2300Plasmid with BClI and BamHI but it did not cut the plasmid can anyone advice me how to do so?

I try to cut them with these enzyme in separat reaction, to linearise the plasmid but it did not work, Although I incubate them for 2hours at 37 heat block.

05 May 2018 9,917 4 View