How can I bevel a glass pipette at 45 ° with a Narishige Microforge for cloning?

15 April 2021 0 2K Report

I need to bevel glass pipettes with an angle of 45 and spike tip with a Narishige microforge. Can anyone give me an advice regarding the position of the pipette in the equipment and heat to apply? Thanks!!

Badges
Science topic

More Alba Ledesma's questions See All

¿Dónde podría buscar fundamentos científicos que verifiquen que la mujer con discapacidad está invisibilizada en el ámbito laboral?

Siendo trabajadora en una asociación de personas con discapacidad, en la actualidad estoy inversa en una investigación sobre "La mujer con discapacidad intelectual en el ámbito laboral" partiendo...

10 March 2024 9,266 2 View

Do my iPSCs have a normal morphology?

Hello everyone, I am starting to grow the SCTi003-A iPSC line for the first time and I am facing some drawbacks. Due to some problems with the first pass, I waited a bit longer than the 7-8 days...

22 February 2024 8,052 2 View

How do I delete a publication that has already been placed on the platform?

09 April 2023 2,170 2 View

What are the risks of sharenting?

More and more adults are sharing content and photos of their children on social networks, but will this have negative consequences? It's a fashion? 📷

02 March 2023 2,473 2 View

How much does it cost to sequence the full genome of a non human eukariotic organism nowadays?

Let's suppose there's an ecology investigation going on. The most relevant species of insects in a mountain range should be sequenced because they want to make a phylogenetic tree using...

16 October 2022 4,954 3 View

Does anyone know if it is possible to buy the mouse-adapted strains of H. pylori?

We want to test a compound against H. pylori, but it has been challenging to find the mouse-adapted strains.

01 June 2022 7,208 2 View

Why are some path coefficients higher than 1 in PLSc?

When calculating PLS consistent algorithm with SmartPLS, some path coefficients could be higher than 1. Why is this? Is there any problem? Does it have a solution?

22 May 2022 1,235 2 View

Why an apparently correct Illumina Sequencing Library only gives 1% yield on a Hiseq550 HighOutput flowcell??

I have generated an Illumina Sequencing Library by nested PCR and size-selected from agarose gel. When measured with Nanodrop, the DNA is clean and of perfect quality. When sequenced with Sanger,...

05 May 2022 4,764 0 View

Cryosection zebrafish larvae WITHOUT fixation?

I need using cryostat with NO fixing embryos. Any suggestion for getting more "robust" sample? I have some tissue problem after sectioning... Thank you so much!

08 March 2022 4,310 0 View

Does any one have experience with Novogene for shotgun metagenomics sequencing?

Hello, I am trying to get a metagenomic analysis and found Novogene whose prices are pretty cheap (almost 1/3 of our university core). Does anyone have any experience with this company? about the...

07 March 2022 8,023 6 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Fabrication of narrow glass capillary (inner diameter 200~500 nm)?

I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?

08 August 2024 8,915 0 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View