Hello everyone ,
I am following a protocol where I treat my cells with 1 .. 2.. or 3 compounds
and I do an atp cell titer glo assay
I noticed that the more compounds my cells receive . the lower their viability is .. which messes up everything
did anyone face this issue before? does it make sense ?
Thanks!
Are you adding the compounds in a solvent, such as ethanol, methanol or DMSO? The solvent could be affecting cell viability.
Hello Adam B Shapiro
thank you for your reply
yes one of my compounds is dissolved in DMSO but I treat the other cells with DMSO vegicle alone to avoid any changes due to DMSO
do any other thoughts come to mind as to what could be causing this ?
The compounds themselves may be mildly cytotoxic. Adding up the mild cytotoxicity of multiple compounds may lead to the loss of viability you are seeing. It is also possible for there to be synergy between compounds in terms of their cytotoxicity, which means that the effect of the combination is greater than the sum of their individual effects.
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