Does the SUMO protease need to inactive after de digestion?

06 June 2019 1 3K Report

Hi everyone,

I have to use the SUMO protease to cleave the SUMO protein from my recombinant protein. After that I will put in contact (ice, one hour) the product of the digestion with the resin resuspended in a buffer. Next, it will be centrifuged and the protein will be in the supernatant.

I would like to know if when I will put in contact the digested product with the resin, it will be necessary to inactivate the enzyme. How can I do that?

Shamsher S. Kanwar

Usually it is better to remove residual or unused protease. The BSA or casien may be added on which residual protease may actually and get consumed

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