Does the Horse serum must heat inactivated for the diffrentition of C2C12 myoblast cells ?

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Does the Horse serum must heat inactivated for the diffrentition of C2C12 myoblast cells ?

Hello, Please can you help me, I need to develop a C2C12 model in my laboratory and I would like to know if I should heat inactivated horse serum. Thank you for your answers! Have a beautiful...

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Valeria De Arcangelis

I never inactivated HS for C2C12

Subhash C. Juneja

People have used new born calf serum as heat inactivated for the presence of complements (to inactivate) whereas, for fetal calf serum, heat inactivation is not necessary for most of cell lines. As Valeria did not inactivated horse serum, may be you can follow that.

Marchand Severine

Thank you for your answers !