Hi, I want to extract essential oils from leaves but I have to delay for a few days due to circumstances. Can I store the leaves for a few days in -20? Does it affect the phytochemicals in the leaves? A lot of people recommended to extract it from fresh leaves but if I have to delay the process is there any method that effective to retain the leave freshness?
Hi, at my lab I have done the same thing, if we will extract many samples simultaneously, at first we collect all samples and keep in -20; generally, to make sure, if there are no degradation occurred during keeping -20, we do extraction directly to a small part of the leaves, and compare the TLC/HPLC profile after keeping in -20., best regards
Storing samples at -20 is safe for a couple of weeks. Best to take mass of sample before freezing as this may be altered when material is thawed.
regards
No, it will not, even if you store for long as far as essential oil is concerned
Hi,
Have you considered effect on the yield of the oil which you may need to report? If I were you I will weigh before storing in other to get the exact percentage yield after extraction.
Hi, sorry I read your letter in late, I think that in -20, the leaves are thawed and can be burned by physical alteration of leaves if store too long, the color of leaves is changing. So, I think the caution recommends to keep at least to -5 for one to two weeks to the freezer and compare the GC profile after storing in -5,sincerely
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