Hi, I need references on how to do the scoring for the extension and severity of lung fibrosis. Also would be great if I know how to do the scoring by my self, can anyone help?
You can score them after stained with Masson's trichrome (Collagen). You may score them based on thickness of the collagen deposition or severity of the fibrosis (semi quantification like 1-4 scale).
Good Luck.
Arul.
Thank you Arul!
Do you know of any published work that has this on their methods?
Hi Carolina, you can score lung fibrosis based on pixel counts of your stained slides:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252890/
http://www.biomedcentral.com/1471-2407/13/179
http://www.pnas.org/content/103/48/18278.full
http://scholar.google.com/scholar?q=%22lung+fibrosis%22+%22pixel+count%22&btnG=&hl=en&as_sdt=1%2C21
Thank you Steingrimur!
I'll check on the references you kindly posted here.
Caution...Masson's is NOT specific for collagen, it also detects BM components. If you want to quantitate collagen specifically, the only histo stain specific for it is picrosirius red, which also can be digitally quantitated under polarized light. Sorry Arul...Carolina, email if you need citations
Check the article
"Biotechniques. 2008 Apr;44(4):507-11, 514-7. doi: 10.2144/000112729.
Standardized quantification of pulmonary fibrosis in histological samples.
Hübner RH1, Gitter W, El Mokhtari NE, Mathiak M, Both M, Bolte H, Freitag-Wolf S, Bewig B"
They use a Modified Scale of the Ashcroft Scale
You can use picrosirius red, which stains collagen red, or you can use Gordon and Sweet's reticular fibre stain, which stains collagen types I and III fibres black. We quantify the collagen based on colour pixels using the image analysis software ImagePro Plus, or you can use a program like Image J.
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